AIIMS Kalyani's 179.82 acres (0.7277 km2) campus is in Saguna, Kalyani, West Bengal, an affluent suburban residential community 50 kilometres (31 mi) north of downtown Kolkata. It is directly north of the NH 34 Connector (the road connects Kalyani with NH 34), directly west of the Jamuna river and about 12 km east of Hooghly river.

The campus consists of mostly housing and academic buildings for the MBBS students, B.Sc (Nursing) students, and staff. Infrastructure (buildings and roads etc.) covers a total area of 57650.21 square meters, which is about 7.92% of the total campus's area. The hospital block is at the southern end of the campus, which is located near the main entrance to the campus. It covers a total area of 22,574.16 square meters. Ayush Block (Ayurveda and Homeopathy) is directly west of the Outpatient department (OPD) building, and only liquid oxygen plant of the institution is located to the west of the Inpatient department (IPD) building. There is only one canteen named "Dharamshala" within the campus, which is located west of Ayush Block.

Seven hostel buildings are located north of the parking lot (for AIIMS staff) and auditorium. The hostel buildings can accommodate a total of 1397 students. The complex of medical college building, nursing college building and laboratory building (medical) is to the north of the hospital building and to the west of the institution's administrative building.